CABRI Guidelines

LABORATORY PROCEDURES FOR GENOMIC LIBRARIES

Part 1: Introduction and general documents

Procedures

Part 2: Request and handling new deposits

Flow diagram

(no standard operating procedures)

Part 3: Maintaining deposits

Procedures

Part 4: Delivery of material to the customer

Procedures

Part 1: Introduction and general documents

Reference Number	Procedure Title
GLI/1998/1	INTRODUCTION

Part 3: Maintaining deposits

Reference Number	Procedure Title
GLI/1998/3/1	PRODUCTION OF HIGH-DENSITY GRIDDED YAC FILTERS
GLI/1998/3/2	PRODUCTION OF YAC CELL POOLS FOR SPHEROPLASTING
GLI/1998/3/3	SPHEROPLASTING YAC POOLS
GLI/1998/3/4	QUALITY CONTROL: PCR ON YAC POOLS
GLI/1998/3/7	PRODUCTION OF HIGH DENSITY GRIDDED PAC/FUGU FILTERS
GLI/1998/3/9	BUFFERS, SOLUTIONS AND REAGENTS
GLI/1998/3/11	96-WELL YAC LIBRARIES: REPLICATION
GLI/1998/3/12	PREPARING 4x4 GLYCEROL PLATES OF YAC CLONES
GLI/1998/3/13	384-WELL E. coli LIBRARIES: CONDENSING FROM 96-WELL AND REPLICATION

Part 4: Delivery of material to the customer

Reference Number	Procedure Title
GLI/1998/4/1	ISSUING PAC POOL AND CLONE REQUESTS
GLI/1998/4/2	ISSUING cFUGU FILTER SETS AND CLONE REQUESTS
GLI/1998/4/3	ISSUING YAC CLONE REQUESTS
GLI/1998/4/4	MAKING UP SETS OF DNA AGAROSE PELLETS FOR ISSUE TO USERS

Guidelines prepared for CABRI by HGMP, December 1998
Page layout by CERDIC
Copyright CABRI, 1998

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